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The RNA for RT-PCR testing was extracted from fresh samples using the STARMag 2019-n Co V kit (Seegene Inc) by means of a liquid-dispensing robot. The SARS-Co V-2 RNA was detected using the Allplex SARS-Co V-2 assay, a multiplex RT-PCR assay that detects 4 SARS-Co V-2 target genes in a single tube. Qualitative assays were performed.
JMIR Public Health Surveill 2024;10:e56926
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Moreover, frailty and multimorbidity evolve as people age, and these patterns can change over time, defining a trajectory. Only a few studies have been found that explored these trajectories [19], using hidden Markov models [23,35], latent class growth analysis [36], and descriptive statistics [37].
JMIR Public Health Surveill 2023;9:e45848
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