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Skip search results from other journals and go to results- 2 JMIR Research Protocols
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Beta-isomerized C-terminal telopeptide (a bone resorption marker) and procollagen 1 N-terminal propeptide (a bone formation marker) will be quantified using a Roche Hitachi Cobas e602 immunoassay analyzer, according to the manufacturer’s guidelines. Hormones (PTH), lipids, glucose and insulin, inflammation markers (C-reactive protein and serum IL-6), and potentially other cardiovascular or health markers will be analyzed according to standard hospital procedures.
JMIR Res Protoc 2021;10(4):e18777
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Blood will be centrifuged (10 min at 3500 rpm, 4°C) and serum and plasma will be immediately stored in aliquots at −80ºc until assayed. In addition, a total of three muscle biopsies from the vastus lateralis will be undertaken in each experimental session. Muscle will be analyzed for insulin signaling proteins, markers of muscle inflammation and atrophy signaling proteins. In total, seven muscle biopsies will be obtained during the study.
JMIR Res Protoc 2016;5(2):e78
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