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Then, participants consumed a fixed liquid meal (Ensure Plus, Abbott Nutrition; 900 kcal in 660 g of fluid: 30% fat, 15% protein, and 55% carbohydrate) from –5 to 0 minutes. To capture GLP-1 and insulin responses to the fixed meal, 2 ml blood samples were drawn at +5, +15, and +30 minutes. Momentary ratings were completed immediately before each blood draw.
JMIR Res Protoc 2025;14:e66554
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Portfolio Diet.app dashboard with key features highlighted, top to bottom: (A) learn tab that has recipes, tipsheets, and videos; (B) daily average Portfolio Diet Score per month; (C) star rewards, a form of reward for logging into the app and for completing the question of the week; (D) current day total Portfolio Diet Score; (E) specific daily messages related to goals; (F) personal favorite meals for easy tracking; (G) subcategory Portfolio Diet Scores with daily targets of 5 points; (H) progress-tracking
JMIR Cardio 2025;9:e58124
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The tubes are centrifuged at 5000 g for 5 minutes at room temperature to pellet the powdered hair. The entire amount (~1 m L) of supernatant is transferred to a clean microcentrifuge tube, and the methanol is removed by evaporation using a stream of air for 6-8 hours at room temperature. The cortisol extract is reconstituted in 100 μL of Salimetric immunoassay cortisol analysis diluent buffer. Samples are assayed in duplicate inter- and intra-assay coefficients of calculated variation.
JMIR Res Protoc 2025;14:e66975
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Sample size calculation was undertaken using procedures provided by the software program G*Power 3 [37]. As per our statistical consultant, the calculation was undertaken to determine adequate power in the determination of the potential effects of the intervention (aim 3), in which a repeated-measures ANOVA with a within-between interaction would be the statistical test used.
JMIR Res Protoc 2025;14:e55475
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